biotinylated lycopersicon esculentum tomato lectin (Vector Laboratories)
Structured Review

Biotinylated Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b+1175/Biotinylated+Lycopersicon+Esculentum+(Tomato)+Lectin+(LEL%2C+TL)/pmc12893876-310-8-13
Average 95 stars, based on 431 article reviews
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1) Product Images from "Co-delivery of synaptogenic and angiogenic nanoparticles in MAP scaffolds enhances post-stroke synapse formation"
Article Title: Co-delivery of synaptogenic and angiogenic nanoparticles in MAP scaffolds enhances post-stroke synapse formation
Journal: Journal of Materials Chemistry. B
doi: 10.1039/d5tb02179k
Figure Legend Snippet: Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato Lectin) and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.
Techniques Used: Staining
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Article Snippet: Then calculated the difference value TA B L E 1 Primary antibodies used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used TNF‐α Escherichia coli‐derived recombinant mouse TNF‐alpha R and D Systems, goat polyclonal, AF‐410‐NA, AB_354479 1:100 Iba‐1 Synthetic peptide corresponding to C‐terminus of Iba1 Wako, rabbit polyclonal, 019‐19741, AB_839504 1:1,000 Cleaved caspase‐3 Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino‐termi‐ nal residues adjacent to (Asp175) in human caspase‐3 Cell Signaling Technology, rabbit polyclonal, 9661, AB_2341188 1:300 Nitrotyrosine Nitrated klh (keyhole limpet hemocyanin) Sigma, rabbit polyclonal, N0409, AB_260745 1:200 RAGE Synthetic peptide, (C)WRKRQP(R/L) EERKAP ESQED(N), corresp. to aa 362‐380 of rat RAGE Abcam, rabbit polyclonal, ab3611, AB_303947 1:200 4HNE Synthetic 4‐Hydroxynonenal modified Keyhole Limpet Kemocyanin (KLH) JalCA, mouse monoclonal, MHN‐100P, AB_1106813 1:50 8‐OHdG 8‐hydroxy‐2′‐deoxyguanosine is produced by reactive oxygen and nitrogen species, including hydroxyl radical and peroxynitrite JalCA, mouse monoclonal, MOG‐100P, Concentration Assay:Article Title: Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2. Article Snippet: .. Primary Antibodies Used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used rat-IgG Antibody recognizes both rat-IgG heavy and light chains Vector Laboratories, rabbit, BA-4000, AB_2336206 1:500 PAR-1 Epitope mapping within amino acids 42–55 of thrombin receptor of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13503, AB_2101175 1:50 PAR-2 Epitope mapping within amino acids 37–50 of PAR-2 of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13504, AB_628101 1:50 PAR-3 Epitope mapping at the C-terminus of PAR- 3 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8209, AB_2101327 1:50 PAR-4 Epitope mapping at the C-terminus of PAR- 4 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8462, AB_2159244 1:50 NeuN Recombinant fragment corresponding to human NeuN aa 1–100 (N terminal) Abcam, rabbit polyclonal, ab104225, AB_10711153 1:500 LEL A stable single subunit glycoprotein contain- ing about 50% arabinose and galactose Vector Laboratories, Recombinant:Article Title: Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2. Article Snippet: .. Primary Antibodies Used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used rat-IgG Antibody recognizes both rat-IgG heavy and light chains Vector Laboratories, rabbit, BA-4000, AB_2336206 1:500 PAR-1 Epitope mapping within amino acids 42–55 of thrombin receptor of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13503, AB_2101175 1:50 PAR-2 Epitope mapping within amino acids 37–50 of PAR-2 of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13504, AB_628101 1:50 PAR-3 Epitope mapping at the C-terminus of PAR- 3 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8209, AB_2101327 1:50 PAR-4 Epitope mapping at the C-terminus of PAR- 4 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8462, AB_2159244 1:50 NeuN Recombinant fragment corresponding to human NeuN aa 1–100 (N terminal) Abcam, rabbit polyclonal, ab104225, AB_10711153 1:500 LEL A stable single subunit glycoprotein contain- ing about 50% arabinose and galactose Vector Laboratories, Purification:Article Title: Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2. Article Snippet: .. Primary Antibodies Used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used rat-IgG Antibody recognizes both rat-IgG heavy and light chains Vector Laboratories, rabbit, BA-4000, AB_2336206 1:500 PAR-1 Epitope mapping within amino acids 42–55 of thrombin receptor of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13503, AB_2101175 1:50 PAR-2 Epitope mapping within amino acids 37–50 of PAR-2 of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13504, AB_628101 1:50 PAR-3 Epitope mapping at the C-terminus of PAR- 3 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8209, AB_2101327 1:50 PAR-4 Epitope mapping at the C-terminus of PAR- 4 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8462, AB_2159244 1:50 NeuN Recombinant fragment corresponding to human NeuN aa 1–100 (N terminal) Abcam, rabbit polyclonal, ab104225, AB_10711153 1:500 LEL A stable single subunit glycoprotein contain- ing about 50% arabinose and galactose Vector Laboratories, Isolation:Article Title: Reduction of intracerebral hemorrhage by rivaroxaban after tPA thrombolysis is associated with downregulation of PAR-1 and PAR-2. 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Primary Antibodies Used Antibody Description of immunogen Source, host species, catalog no., RRID Concentration used rat-IgG Antibody recognizes both rat-IgG heavy and light chains Vector Laboratories, rabbit, BA-4000, AB_2336206 1:500 PAR-1 Epitope mapping within amino acids 42–55 of thrombin receptor of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13503, AB_2101175 1:50 PAR-2 Epitope mapping within amino acids 37–50 of PAR-2 of human origin Santa Cruz Biotechnology, mouse monoclonal, sc-13504, AB_628101 1:50 PAR-3 Epitope mapping at the C-terminus of PAR- 3 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8209, AB_2101327 1:50 PAR-4 Epitope mapping at the C-terminus of PAR- 4 of mouse origin Santa Cruz Biotechnology, goat polyclonal, sc-8462, AB_2159244 1:50 NeuN Recombinant fragment corresponding to human NeuN aa 1–100 (N terminal) Abcam, rabbit polyclonal, ab104225, AB_10711153 1:500 LEL A stable single subunit glycoprotein contain- ing about 50% arabinose and galactose Vector Laboratories, Immunofluorescence:Article Title: Nicotinamide riboside rescues angiotensin II–induced cerebral small vessel disease in mice Article Snippet: The nuclei were counterstained with 4′‐6‐diamidino‐2‐phenylindole (DAPI; Santa Cruz Biotechnology). .. To visualize brain blood vessels, biotinylated Lycopersicon esculentum (tomato) lectin (LEL, TL) (1:200; Vector Laboratories, |